Fibronectin From Oviductal Cells Fluctuates During the Estrous Cycle and Contributes to Sperm-Oviduct Interaction in Cattle.
Osycka-Salut, C. E., Castellano, L., Fornes, D., Beltrame, J. S., Alonso, C. A. I., Jawerbaum, A., Franchi, A., Diaz, E. S. and Perez Martinez, S.
Laboratorio de Biotecnologias Reproductivas y Mejoramiento Genetico Animal (IIB-INTECH Dr. Rodolfo Ugalde, CONICET/UNSAM), Buenos Aires, Argentina.
Laboratorio de Biologia de la Reproduccion en Mamiferos, (CEFYBO-CONICET/UBA), Buenos Aires, Argentina.
Laboratorio de Reproduccion y Metabolismo, (CEFYBO-CONICET/UBA), Buenos Aires, Argentina.
Laboratorio de Fisiologia y Farmacologia de la Reproduccion, (CEFYBO-CONICET/UBA), Buenos Aires, Argentina.
Laboratorio de Fisiopatologia de la Prenez y el Parto, (CEFYBO-CONICET/UBA), Buenos Aires, Argentina.
Laboratorio de Biologia de la Reproduccion, Facultad de Ciencias de la Salud, Universidad de Antofagasta, Antofagasta, Chile.
During the passage of sperm through the oviduct, spermatozoa bind to the oviductal epithelium and form the oviductal reservoir. This interaction keeps the fertilizing capacity of sperm until ovulation-associated signals induce sperm release from the oviductal epithelium, allowing the transit of spermatozoa to the fertilization site. Fibronectin is a glycoprotein from the extracellular matrix that binds to alpha5beta1 receptors. Fibronectin has been found to be expressed in the oviduct, whereas alpha5beta1 has been found to be expressed in the sperm of different species. Fibronectin is involved through alpha5beta1 in sperm functions. The aim of this work was to study the participation of oviductal fibronectin in the regulation of the sperm-oviduct interaction in cattle. We found that oviductal epithelial cells differentially expressed all mRNA splice variants of fibronectin during the estrous cycle. Fibronectin was localized in the apical region of oviductal epithelial cells and fibronectin levels in the oviductal fluid fluctuated during the estrous cycle. Also, bovine spermatozoa expressed alpha5beta1. Using in vitro sperm-oviduct co-cultures, we found that spermatozoa were attached to the oviductal epithelium through alpha5beta1. The incubation of co-cultures with fibronectin induced sperm release from the oviductal cells through alpha5beta1. The sperm population released from oviductal cells by fibronectin was enriched in motile and capacitated spermatozoa. Based on our in vitro culture system results, we propose that fibronectin and alpha5beta1 are involved in the sperm-oviduct interaction. Also, an increase in fibronectin levels in the oviductal fluid during the pre-ovulatory period may promote sperm release from the oviductal epithelium in cattle. J. Cell. Biochem. 9999: 1-14, 2017. (c) 2017 Wiley Periodicals, Inc.
Journal of Cellular Biochemistry : en prensa (2017)